Agdia announces the commercialization of a speedy , substance abuser - friendly , DNA - free-base assay on their AmplifyRP ® XRT platform to find Fusarium oxysporum .
The Fusarium oxysporum species is a omnipresent fungal inhabitant of soils throughout the world . member of this species complex are commonplace in the microbic community of works rhizospheres of civilize crops , monocots , and dicot , roam from tropic to temperate climate . While many strains of F. oxysporum are harmless saprobe , others are considered significant plant pathogen , even confine factors to crop production .
The pathogens in this genus have an all-embracing host mountain chain at the mintage level . However , single strains of F. oxysporum , lie with independently as a forma specialis ( f. sp . ; plural : formae speciales , ff . spp . ) or special form(s ) , exhibit extremely selective host pathogenicity , typically taint no more than a few metal money of plant . Collectively , there are more than 100 recognized formae speciales of F. oxysporum , causing vascular wilt disease or root and crown rot on several economically authoritative crops , admit Asparagus officinales , banana tree , cannabis , chrysanthemum , uncouth noodle , cotton , lettuce , melon , soybean , strawberry mark , tomato and several cultivated member of Orchidaceae .
Amongst the symptomology induced by F. oxysporum , vascular wilts are most coarse . Fungal mycelia are soil - borne and enter the plant via raw possible action or damage tissue paper in the root word organisation . After that , the pathogen invades the xylem tissue and grow acropetally , overload xylem vas , producing microconidia , and impeding the up bowel movement of weewee and nutrients . Infected plants display severe chlorosis , one-sided wilting , and overall collapse . Plants infected with strains inducing crown and root rots expose progressive mortification of the root system and aerial basal tissue . Mycelia do not get across the vascular tissue during this pathogenesis , but plant expiry occurs nevertheless due to the prostration of the ascendent system . How sieve differentiate host and colonization strategy is not well characterise . However , it is hypothesized that the fungi regulate these processes based on legion plant human body and gene - for - gene interactions . The inquiry investigating this genomic basis is ongoing .
Fusarium oxysporum is spread retentive distance via the movement of septic plant materials such as cuttings , transplants , roots , medulla oblongata , and corms . moreover , spore and mycelium can be transported in infested soil on shoes and wearable , tools , equipment , and in aerofoil water supply . Its persistent nature and multiple avenues of dissemination make eradication of this pathogen impossible within affected orbit . Fungicide applications are impractical in field - grown crops due to large acreages and the limitations of fungicide movement through territory . Drenches are apply in greenhouse crops with success ; however , crop such as cannabis are subject to organic growing standards , precluding the use of synthetic fungicides . Genetic underground is efficient in craw such as tomatoes but is not wide available amongst hosts of F. oxysporum strains . Exclusion of the pathogen through rapid containment is the most efficacious management strategy . This make the diagnostic testing of plant material paramount to successful disease management programs .
Making testing simpleAgdia ’s unexampled AmplifyRP ® XRT check for signal detection of Fusarium oxysporum is establish on recombinase polymerase amplification ( RPA ) . This technology promotes the speedy amplification and detection of nucleic back breaker targets , deoxyribonucleic acid or RNA , while keep a single operating temperature of 39 – 42 ° C . The AmplifyRP ® XRT products achieve target sensitivity and specificity like to PCR , while make readable vantage over the laboratory - based engineering science . AmplifyRP ® XRT products do not require a nucleic acid purification stair ; crude sample distribution extracts are prepared using a simple extraction buffer and test directly . This makes the testing process dim-witted and saves the end - exploiter valuable time . moreover , this facilitates the implementation of this technology at removed locations with limited resources . When paired with Agdia ’s AmpliFire ® isothermal fluorometer , the XRT system is a rapid , user - friendly tool that can be implemented in the landing field or the lab by personnel department with limited experience in molecular diagnostics .
Agdia states their new check was screened against multiple formae speciales of Fusarium oxysporum , include cannabis , chrysanthemi , fragariae , latucae , lycopersici , niveum , and vasinfectum , detecting all rightful positives . what is more , a high spirit level of specificity was observed with an extensive exclusivity panel of multiple metal money of Fusarium other than F. oxysporum and additional fungal and oomycete pathogens , admit Phytophthora spp , Pythium spp . , Rhizoctonia solani , and Verticillium dahliae . predisposition for this assay is like to that observe with the published qPCR check and greater than the published conventional PCR assay to which it was compared . This product was make grow to test crown , leaf , petiole , origin and stem tissue . This assay take an endogenous internal desoxyribonucleic acid control .
Agdia now declare oneself AmplifyRP ® XRT products to notice several important bacterial , fungous , viral , and virusoid pathogen across several marketplace , include grape , cannabis , decorative , spud , and tomato . “ We are working to develop a comprehensive portfolio of easy - to - manipulation diagnostic products on our AmplifyRP ® XRT platform . client demand for check that can be implemented across a spacious spectrum of applications has driven our output . We continue to focalize on merchandise development for effected customers and burgeoning markets , such as the cannabis diligence , ” said Robert Emmitt , Domestic Account Manager , Agdia .
For more selective information : Agdia Inc.52642 County Road 1Elkhart , IN 46514phone 1 - 574 - 264 - 2615fax 1 - 574 - 264 - 2153[email protected]www.agdia.com
